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(a) Scheme of GRN analysis <t>using</t> <t>Fluidigm</t> <t>C1</t> scRNA-seq data. Dashed-line boxes indicate the TF subnetwork, which was divided into overlapping communities. Each TF is connected to its first-edge targets to define “overall communities” (OCs). (b) Heatmap showing the enrichment of representative OCs across integrated clusters. Color scale represents z-scored enrichment values. (c) UMAP plots showing representative OC enrichment patterns, colored by cluster identity as in (d) with intensity indicating enrichment levels. (d) Schematic representation of NCC lineage trajectories annotated with OCs and key TFs.
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(a) Scheme of GRN analysis <t>using</t> <t>Fluidigm</t> <t>C1</t> scRNA-seq data. Dashed-line boxes indicate the TF subnetwork, which was divided into overlapping communities. Each TF is connected to its first-edge targets to define “overall communities” (OCs). (b) Heatmap showing the enrichment of representative OCs across integrated clusters. Color scale represents z-scored enrichment values. (c) UMAP plots showing representative OC enrichment patterns, colored by cluster identity as in (d) with intensity indicating enrichment levels. (d) Schematic representation of NCC lineage trajectories annotated with OCs and key TFs.
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(a) Scheme of GRN analysis <t>using</t> <t>Fluidigm</t> <t>C1</t> scRNA-seq data. Dashed-line boxes indicate the TF subnetwork, which was divided into overlapping communities. Each TF is connected to its first-edge targets to define “overall communities” (OCs). (b) Heatmap showing the enrichment of representative OCs across integrated clusters. Color scale represents z-scored enrichment values. (c) UMAP plots showing representative OC enrichment patterns, colored by cluster identity as in (d) with intensity indicating enrichment levels. (d) Schematic representation of NCC lineage trajectories annotated with OCs and key TFs.
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(a) Scheme of GRN analysis using Fluidigm C1 scRNA-seq data. Dashed-line boxes indicate the TF subnetwork, which was divided into overlapping communities. Each TF is connected to its first-edge targets to define “overall communities” (OCs). (b) Heatmap showing the enrichment of representative OCs across integrated clusters. Color scale represents z-scored enrichment values. (c) UMAP plots showing representative OC enrichment patterns, colored by cluster identity as in (d) with intensity indicating enrichment levels. (d) Schematic representation of NCC lineage trajectories annotated with OCs and key TFs.

Journal: bioRxiv

Article Title: Hox – Meis -relayed topographical genetic switch underlies cardiopharyngeal neural crest diversification, revealed by multimodal analysis

doi: 10.1101/2025.11.09.687497

Figure Lengend Snippet: (a) Scheme of GRN analysis using Fluidigm C1 scRNA-seq data. Dashed-line boxes indicate the TF subnetwork, which was divided into overlapping communities. Each TF is connected to its first-edge targets to define “overall communities” (OCs). (b) Heatmap showing the enrichment of representative OCs across integrated clusters. Color scale represents z-scored enrichment values. (c) UMAP plots showing representative OC enrichment patterns, colored by cluster identity as in (d) with intensity indicating enrichment levels. (d) Schematic representation of NCC lineage trajectories annotated with OCs and key TFs.

Article Snippet: To elucidate these GRNs, we utilized additional scRNA-seq datasets from EYFP + NCCs isolated at E11.5, E12.5, E14.5 and E17.5 from Wnt1-Cre;R26R-EYFP mouse hearts, generated using the Fluidigm C1 platform.

Techniques: